ALU PCR LAB
Overview
We conducted the lab to find the amount of people in our class with the alu insert (a genome which occurs in both human and primate DNA). People are either homozygous, which means that both of their parents have (or don't have) the alu insert, or heterozygous, which means that one parent has the alu insert and one does not.
Procedure
- Swirl 10 ml of saline solution in mouth
- Spit solution into cup
- Put 1000ul of solution from cup into 1.5 ml microfuge tube
- Put solution into centrifuge
- Pour off supernatant leaving 100 ul covering cell pellet
- Put 50 ul of cell suspension into chelex.
- Put on heat block for 10 minutes.
- Release pressure from tube and put in centrifuge.
- put 50ul of supernatant in new microfuge tube.
- Pipet 20 ul of master mix, 20 ul of primer mix, and 10 ul of DNA into new PCR tube
- Place in thermal cycler
Gel preparation
- 50 ml of TAE and 1 gram of agarose
- Heat solution until desolved.
- When cold, pour into mold.
- 20 ml of DNA into new tube with 4 ml loading dye
- Centrifuge and load 20 ul into well.
- Record lane and gel number
MAterials
- 0.9% Saline Solution
- Micropipette Tips
- Waste Container
- Water
- PCR Tubes
- Molds
- DNA
- Chelex
- Primer Mix
- Micropipettes tips
- Centrifuge
- Microfuge Tubes
- Agarose
- Gel Chambers
- Load Dye
- Racks
- Master Mix
Personal result
I am homozygous, which means that both of my parents has the alu gene and one does not. I was the ninth row... xD